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Registros recuperados : 16 | |
6. | | GONÇALVES, D.; GABRIEL, J. E.; MADEIRA, H. M. F.; SCHUHLI, G. S. e; VICENTE, V. A. New method for early detection of two random amplified polymorphic DNA (RAPD) groups of Staphylococcus aureus causing bovine mastitis infection in Paraná State, Brazil. Brazilian Archives of Biology and Technology, v. 53, n. 2, p. 353-360, Mar./Apr. 2010. Biblioteca(s): Embrapa Florestas. |
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8. | | SILVA, F. B.; AUER, C. G.; KLISIOWCZ, D. R.; PIMENTEL, I. C.; VICENTE, V. A. Variabilidade de isolados de Armillaria sp da Região Sul do Brasil. In: CONGRESSO BRASILEIRO DE MICOLOGIA, 6., 2010, Brasília, DF. Anais. São Paulo: Sociedade Brasileira de Micologia, 2010. 1 CD-ROM. Biblioteca(s): Embrapa Florestas. |
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9. | | GRUNENNVALDT, R. L.; DEGENHARDT-GOLDBACH, J.; TOMASI, J. de C.; SANTOS, G. D. dos; VICENTE, V. A.; DESCHAMPS, C. Bacillus megaterium: an endophytic bacteria from callus of Ilex paraguariensis with growth promotion activities. Biotecnología Vegetal, v. 18, n. 1, p. 3-13, ene./mar. 2018. Biblioteca(s): Embrapa Florestas. |
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11. | | SOUZA, R. C.; CANTÃO, M. E.; NOGUEIRA, M. A.; VANSCONCELOS, A. T. R.; BARCELLOS, F. G.; VICENTE, V. A.; HUNGRIA, M. Diversidade funcional de genes com potencial biotecnológico em um solo agrícola no Norte do Paraná. In: CONGRESSO BRASILEIRO MICROBIOLOGIA, 27., SIMPÓSIO IBEROAMERICANO SOBRE MICRO-ORGANISMOS FOTOSSINTETIZANTES, 2.; SIMPÓSIO BRASILEIRO DE MICOBACTÉRIAS, 15.; SIMPÓSIO DE FERMENTAÇÃO ALCOÓLICA, 2.; BRAZILIAN MICROBIOME WORKSHOP; BRAZILIAN MICROBIOME PROJECT MEETING, 1.; SIMPÓSIO DE COLEÇÕES DE CULTURA, 4.; MINI-SIMPÓSIO SOBRE NEW DELHI METALO-BETA-LACTAMASE-1 (NDM-1); 2013, NATAL. Anais... [São Paulo]: Sociedade Brasileira de Microbiologia, 2013. Biblioteca(s): Embrapa Soja. |
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13. | | SOUZA, R. C.; CANTÃO, M. E.; NOGUEIRA, M. A.; VASCONCELOS, A. T. R.; GOMES, R. R.; VICENTE, V. A.; HUNGRIA, M. Triagem de hidrolases por metagenômica de solos agrícolas do Norte do Paraná. In: ENCONTRO NACIONAL DE MICROBIOLOGIA AMBIENTAL, 14., João Pessoa, 2014. Anais... Biblioteca(s): Embrapa Soja. |
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14. | | TOMASI, J. de C.; DEGENHARDT-GOLDBACH, J.; GRUNENNVALDT, R. L.; SANTOS, G. D. dos; VICENTE, V. A.; FRANCISCON, L.; BONA, C.; QUOIRIN, M. In vitro establishment of shoot meristems of Ilex paraguariensis and identification of endophytic bacteria. Journal of Forestry Research, v. 30, n. 5, p. 1765-1777, Oct. 2019. Biblioteca(s): Embrapa Florestas. |
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15. | | COSTA, F. de F.; SOUZA, R. C.; VOIDALESKI, M. F.; BOMBASSARO, A.; CANDIDO, G. Z.; SILVA, N. M. da; ROBL, D.; MORENO, L. F.; WEISS, V. A.; RAITTZ, R. T.; CASTRO, M. A.; GOMES, R. R.; BITTENCOURT, J. V. M.; HOOG, G. S. de; HUNGRIA, M.; VICENTE, V. A. New Insights on Environmental Occurrence of Pathogenic Fungi Based on Metagenomic Data from Brazilian Cerrado Biome. Brazilian Archives of Biology and Technology, v. 65, e22210097, 2022. 10 p. Biblioteca(s): Embrapa Soja. |
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16. | | LIMA, B. J. F. de S.; VOIDALESKI, M. F.; GOMES, R. R.; FORNARI, G.; SOARES, J. M. B; BOMBASSARO, A.; SCHNEIDER, G. X.; SOLEY, B. da S.; AZEVEDO, C. de M. P. e S. de; MENEZES, C.; MORENO, L. F.; ATTILI-ANGELIS, D.; KLISIOWICZ, D. do R.; DE HOOG, S.; VICENTE, V. A. Selective isolation of agents of chromoblastomycosis from insect-associated environmental sources. Fungal Biology, v. 124, n. 3-4, p. 194-204, 2020. Biblioteca(s): Embrapa Meio Ambiente. |
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Registros recuperados : 16 | |
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Registro Completo
Biblioteca(s): |
Embrapa Florestas. |
Data corrente: |
13/11/2019 |
Data da última atualização: |
13/11/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
TOMASI, J. de C.; DEGENHARDT-GOLDBACH, J.; GRUNENNVALDT, R. L.; SANTOS, G. D. dos; VICENTE, V. A.; FRANCISCON, L.; BONA, C.; QUOIRIN, M. |
Afiliação: |
Jéssica de Cássia Tomasi, UFPR; JULIANA DEGENHARDT GOLDBACH, CNPF; Renata Lucia Grunennvaldt, UFPR; Germana Dávila dos Santos, UFPR; Vania Aparecida Vicente, UFPR; LUZIANE FRANCISCON, CNPF; Cleusa Bona, UFPR; Marguerite Quoirin, UFPR. |
Título: |
In vitro establishment of shoot meristems of Ilex paraguariensis and identification of endophytic bacteria. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Journal of Forestry Research, v. 30, n. 5, p. 1765-1777, Oct. 2019. |
DOI: |
10.1007/s11676-018-0763-x |
Idioma: |
Inglês |
Conteúdo: |
Yerba mate (Ilex paraguariensis A. St.-Hil.) is a species of great economic, social and environmental importance for the southern regions of Brazil, Uruguay and Argentina. Currently the most diverse products are obtained from mate leaves, including mate tea. The objective of this study was to establish shoot meristem cultures (meristematic dome and a few primordia) of elite clones and identify the endophytic bacteria present in the explants. We tested the effect of clones (F1, F2, A03 and A07), culture media (MS, 1/2MS, 1/4MS and WPM), cytokinins (kinetin, BA and 2iP), activated charcoal (1, 2 and 3 g L-1), and disinfecting agent (sodium hypochlorite and mercuric chloride) on in vitro establishment. F1 and F2 clones were the most responsive for shoot meristem in vitro culture. WPM medium supplemented with 8.8 µM 2iP, 0.2 µM NAA and 3 g L-1 activated charcoal was the most suitable for the in vitro establishment of the F1 clone. No phytotoxic effect of the disinfectant was observed and some meristems sprouted. The isolated endophytic bacterium was identified for the first time in yerba mate as Agrobacterium larrymoorei. To conclude, we were able to establish in vitro culture of yerba mate using meristems as explants but the tissues were not free of endophytic microorganisms which could interfere with explant response. |
Palavras-Chave: |
2iP; Activated charcoal; Endophytic bacteria; Erva mate. |
Thesagro: |
Cultura de Tecido; Ilex Paraguariensis; Oxidação. |
Thesaurus NAL: |
Agrobacterium larrymoorei; Oxidation; Tissue culture; Yerba mate. |
Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
Marc: |
LEADER 02375naa a2200349 a 4500 001 2114253 005 2019-11-13 008 2019 bl uuuu u00u1 u #d 024 7 $a10.1007/s11676-018-0763-x$2DOI 100 1 $aTOMASI, J. de C. 245 $aIn vitro establishment of shoot meristems of Ilex paraguariensis and identification of endophytic bacteria.$h[electronic resource] 260 $c2019 520 $aYerba mate (Ilex paraguariensis A. St.-Hil.) is a species of great economic, social and environmental importance for the southern regions of Brazil, Uruguay and Argentina. Currently the most diverse products are obtained from mate leaves, including mate tea. The objective of this study was to establish shoot meristem cultures (meristematic dome and a few primordia) of elite clones and identify the endophytic bacteria present in the explants. We tested the effect of clones (F1, F2, A03 and A07), culture media (MS, 1/2MS, 1/4MS and WPM), cytokinins (kinetin, BA and 2iP), activated charcoal (1, 2 and 3 g L-1), and disinfecting agent (sodium hypochlorite and mercuric chloride) on in vitro establishment. F1 and F2 clones were the most responsive for shoot meristem in vitro culture. WPM medium supplemented with 8.8 µM 2iP, 0.2 µM NAA and 3 g L-1 activated charcoal was the most suitable for the in vitro establishment of the F1 clone. No phytotoxic effect of the disinfectant was observed and some meristems sprouted. The isolated endophytic bacterium was identified for the first time in yerba mate as Agrobacterium larrymoorei. To conclude, we were able to establish in vitro culture of yerba mate using meristems as explants but the tissues were not free of endophytic microorganisms which could interfere with explant response. 650 $aAgrobacterium larrymoorei 650 $aOxidation 650 $aTissue culture 650 $aYerba mate 650 $aCultura de Tecido 650 $aIlex Paraguariensis 650 $aOxidação 653 $a2iP 653 $aActivated charcoal 653 $aEndophytic bacteria 653 $aErva mate 700 1 $aDEGENHARDT-GOLDBACH, J. 700 1 $aGRUNENNVALDT, R. L. 700 1 $aSANTOS, G. D. dos 700 1 $aVICENTE, V. A. 700 1 $aFRANCISCON, L. 700 1 $aBONA, C. 700 1 $aQUOIRIN, M. 773 $tJournal of Forestry Research$gv. 30, n. 5, p. 1765-1777, Oct. 2019.
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